Three-time defending champion Kei Nishikori successfully defended his title, defeating Taylor Fritz in the final, 6–4, 6–4 to win the singles title at the 2016 Memphis Open. Notably, this was only Fritz' third career event on the ATP Tour.
The top four seeds receive a bye into the second round.
Kei Nishikori (champion)
Steve Johnson (second round) Donald Young (quarterfinals) Sam Querrey (semifinals) Denis Kudla (second round).svg/1280px-Flag_of_Australia_(converted).svg.png){kind=small}
Sam Groth (first round) John Millman (second round)
Damir Džumhur (second round)