Serena Williams defeated Maria Sharapova in the final, 4–6, 6–3, 6–0 to win the women's singles tennis title at the 2013 Miami Open. It was her record sixth Miami Open title, and Sharapova's fifth runner-up finish without winning the title.
Agnieszka Radwańska was the defending champion, but lost in the semifinals to Williams.
All seeds receive a bye into the second round.
Serena Williams (champion)
Victoria Azarenka (withdrew because of a right ankle injury)
Maria Sharapova (final)
Agnieszka Radwańska (semifinals)
Li Na (quarterfinals)
Angelique Kerber (third round)
Petra Kvitová (third round)
Sara Errani (quarterfinals)
Caroline Wozniacki (third round)
Marion Bartoli (second round, retired because of a left foot injury) Nadia Petrova (third round)
Ana Ivanovic (fourth round)
Dominika Cibulková (fourth round) Maria Kirilenko (third round) Roberta Vinci (quarterfinals) Sloane Stephens (fourth round) Lucie Šafářová (second round) Ekaterina Makarova (second round) Venus Williams (third round, withdrew because of a lower back injury)
Carla Suárez Navarro (third round) Klára Zakopalová (fourth round) Jelena Janković (semifinals) Anastasia Pavlyuchenkova (second round) Julia Görges (second round) Varvara Lepchenko (third round)
Tamira Paszek (second round) Mona Barthel (second round)
Sorana Cîrstea (fourth round) Elena Vesnina (third round).svg/1280px-Flag_of_Belgium_(civil).svg.png){kind=small}
Kirsten Flipkens (quarterfinals) Yanina Wickmayer (second round) Alizé Cornet (fourth round) Sílvia Soler Espinosa (qualified).svg/1280px-Flag_of_Switzerland_(Pantone).svg.png){kind=small}
Stefanie Vögele (qualified) Lauren Davis (qualifying competition, lucky loser) Olga Puchkova (first round) Camila Giorgi (moved to main draw)
Misaki Doi (first round) Karolína Plíšková (qualified) Melanie Oudin (first round) María Teresa Torró Flor (first round) Lara Arruabarrena (first round)
Elina Svitolina (qualifying competition) Jana Čepelová (qualified)
Yulia Putintseva (qualified)
Mandy Minella (first round)
Melinda Czink (qualified)
Donna Vekić (qualified) CoCo Vandeweghe (qualifying competition) Vesna Dolonc (first round) Tatjana Malek (qualifying competition)
Maria João Koehler (first round) Galina Voskoboeva (qualifying competition) Kristýna Plíšková (qualifying competition).svg/1280px-Flag_of_Australia_(converted).svg.png){kind=small}
Anastasia Rodionova (first round) Mirjana Lučić-Baroni (first round) Nina Bratchikova (qualifying competition)