El FA Trophy 1990-91 fue la vigésimo segunda temporada del FA Trophy .
Los equipos que dieron pases directos a esta ronda son Barrow, Colchester United, Barnet, Runcorn, Macclesfield Town, Kettering Town, Welling United, Yeovil Town, Sutton United, Merthyr Tydfil, Wycombe Wanderers, Cheltenham Town, Telford United, Kidderminster Harriers, Northwich Victoria, Altrincham, Stafford Rangers, Slough Town, Bath City, Gateshead, Farnborough Town, Aylesbury United, Dartford, Wokingham Town, Redbridge Forest, Billingham Synthonia, Woking, Dover Athletic, Hyde United, Leek Town y Gretna.