The 2012 CNGvitall Prague Open, known also as 2012 CNGvitall Prague Open by Noncore for sponsorship reasons, was a professional tennis tournament played on clay courts. It was the 19th edition of the tournament which is part of the 2012 ATP Challenger Tour. It took place in Prague, Czech Republic between 7 and 13 May 2012.
The following players received wildcards into the singles main draw:
Jan Blecha
Mate Pavić Jiří VeselýThe following players received entry as a special exempt into the singles main draw:
The following players received entry from the qualifying draw:
The following player received entry from a Lucky loser spot:
Axel Michon
Horacio Zeballos def. 
Martin Kližan, 1–6, 6–4, 7–6(8–6). Lukáš Rosol / Horacio Zeballos def. Martin Kližan / Igor Zelenay, 7–5, 2–6, [12–10].
